Antibiofilm activity against Staphylococcus aureus and content analysis of Taraxacum Officinale phenolic extract

Taraxacum Officinale, commonly called dandelion, is herbaceous perennial belonging to the family of Asteraceae, having good antibacterial effects which are related its phenolic substances. In this study, effect contents as well antibiofilm activity against Staphylo- coccus aureus extract from T. Officinale were evaluated in vitro. With 70% metha- nol-water (v/v) a solvent, dandelion was extracted by ultrasonic assisted extraction method. Subsequent identification and quantification phenol carried out using High Performance Liquid Chromatography (HPLC). The minimum inhibitory concentration anti- bacterial kinetic curve analyzed spectrophotometry. Changes extracellular alkaline phosphatase (AKP) contents, electrical conductivity, intracellular protein DNA S. after action determined study on permeability cell wall membrane. results showed that chlorogenic acid (1.34 mg/g) present higher concentration, followed lute- olin (1.08 mg/g), ferulic (0.22 caffeic (0.21 rutin (0.19 extract. (MIC) 12.5 mg/mL. analysis mainly exponential growth phase. After applying extract, signifi- cantly inhibited entering into decay phase early. Furthermore, AKP aureus, conductivity all increased. also affected normal reproduction aureus. These suggest has an mechanism destroy integrity walls membranes.